fluorescent situ hybridisation (fish) probes  (MetaSystems inc)

Journal: Hernia

Article Title: Are late hernia mesh complications linked to Staphylococci biofilms?

doi: 10.1007/s10029-022-02583-0

Figure Lengend Snippet: Confocal microscopy images of PNA-FISH labelled S. aureus (green) and coagulase-negative staphylococci (red). Cell nuclei of tissue stained with DAPI (blue). Scale bar 15 µm. A Optilene mesh from an open inguinal hernia repair excised for chronic pain and mesh shrinkage. Image shows biofilm on a mesh strand (blue autofluorescence of the mesh) containing S. aureus and coagulase-negative staphylococci within the biofilm. PCR results were concordant for both bacteria. B Ultrapro mesh from an open inguinal hernia repair excised for chronic pain. Image shows a biofilm containing abundant S. aureus colonies and fewer coagulase-negative staphylococci. PCR results were concordant for both bacteria . C TiMesh plug from an open inguinal hernia repair excised for chronic pain. Image shows biofilm on a mesh strand (blue autofluorescence of the mesh) containing predominantly coagulase-negative staphylococci . PCR results were positive for S. epidermidis . D Atrium mesh (pale green autofluorescence) from a laparoscopic TEP repair excised for recurrence. Image shows biofilm containing both S. aureus and coagulase-negative staphylococci. PCR results were concordant for both bacteria

Article Snippet: Each mesh specimen was stained with peptide nucleic acid fluorescence in situ hybridisation (PNA-FISH) probes from AdvanDx (OpGen®, Denmark) specific for S. aureus and coagulase-negative staphylococci rRNA using the manufacturer’s protocol.

Techniques: Confocal Microscopy, Staining, Bacteria